Developing Purification Strategies for the Agilent 1260 Infinity II Preparative LC/MSD System


Peak-based fraction collection triggered by a UV signal threshold leads to
satisfactory results in most cases. Some samples and applications, however,
require special attention when fraction collection parameters are set. This
Technical Overview describes different strategies on how to achieve best
collection results using different settings on an Agilent 1260 Infinity II Preparative
LC/MSD System. UV and mass-based fraction collection, fraction pooling, as well
as recovery collection are discussed in detail to provide knowledge that can be
turned into successful and confident fraction collection.

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