Metabolic discovery platform: “Seahorse-Bravo”

The regulation of energy metabolism is very complex and involves a large number of signaling pathways and
molecular effectors. Recent development in biology revealed that cancer cells can rewire most metabolic pathways,
revealing novel means of bioenergetic regulation (Figure 1). For instance, it was shown that C-MYC oncogene controls
glutamine-supported cellular respiration and lipid synthesis or that glycolysis bottlenecking by PKM2 promotes de
novo serine biosynthesis. With the recently ascertained role of mitochondria in cancer biology, the search for novel
regulators of mitochondrial respiration was performed using genetic means, as CRISPR-CAS9 or shRNA libraries, or
pharmacological means such as drug-libraries. There is a growing need in academia and industry to dispose
technology solutions enabling the discovery of mitochondrial respiration regulators (genes or compounds) using
standardized and reproducible high-precision methods. The aim of this unprecedented CELLOMET-Agilent
collaboration is to evaluate the quality and the efficiency of coupling the Agilent Seahorse XF96 Analyzer
extracellular flux analyzer with the Agilent Bravo Automated Liquid Handling Platform.

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