Fully Automated Characterization of Monoclonal Antibody Charge Variants Using 4D-LC/MS

This application note describes the fully automated and in-depth characterization of monoclonal antibody (mAb) charge variants by four-dimensional liquid chromatography/mass spectrometry (4D-LC/MS) using the Agilent InfinityLab 2D‑LC Solution and the Agilent 6545 LC/Q-TOF system. Charge variants resolved by cation-exchange chromatography (CEX) are collected in loops installed on a multiple heart-cutting valve and consecutively subjected to online desalting, denaturation, reduction, and tryptic digestion prior to LC/MS-based peptide mapping. Za odpiranje dokumenta
kliknite tukaj …

Comments are closed.