Best Practices for Using an Agilent LC System

This technical note describes best practices for using an Agilent LC system. Contents Housekeeping 2 How to deal with solvents 2 How to prepare samples 3 Daily / Weekly tasks 4 Power up / Shut-down the system 5 Power-up the system 5 Shut-down the system 6 Recommendations for degassers 7 Recommendations for pumps 8 Recommendations for Pumps with MCGV 8 Seal Wash (usage mandatory when installed) 9 Recommendations for samplers 13 Recommendations for pumps and samplers with optional inline filter 14 Recommendations for columns 15 Recommendations for detectors 16 Additional Information for 1290 Infinity and 1290 Infinity II Pumps 17

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