Best practices when working with Agilent HPLC system


Agilent Technologies
Best Practices
Solvents:
• Use clean borosilicate bottles only
• Prepare solvent volume to be used up within 1 – 2 days
• Use only HPLC- grade or better solvents filtered through 0.2 µm filters
• Label bottles correctly
• Reduce risk of exposure to direct sun light by using brown bottles
Powering up the pump:
• Use fresh solvents and solvent bottles
• Inspect solvent bottles and inlet filters for damages or coloring
• Check function and settings of the seal wash
Preparing the pump:
• Purge the pump to remove old solvent, salt deposits or particulate
matter
• Condition the system with appropriate conditioning method
Daily tasks:
• Replace water based mobile phases daily
• Replace organic mobile phases every 2nd day
• Check seal wash solvent
• Use the Conditioning function with composition of your application
Weekly tasks:
• Change seal wash solvent and bottle
• Inspect solvent filters
• Flush all channels to remove salt deposits and particulate matter
• Check system backpressure and change filters if necessary
Pump shut-down:
• Flush all channels to remove salt deposits and particulate matter
• Flush the system with appropriate storage solvent
• Power down the system
*01200-90091*
01200-90091
Part Number:
01200-90091
Edition: 04/2015
Printed in Germany
© Agilent Technologies, Inc 2015
Agilent Technologies, Inc
Hewlett-Packard-Strasse 8
76337 Waldbronn, Germany
1290 Infinity Pump Quick Reference Sheet
Seal Wash (usage mandatory when installed)
Benefits of Seal Wash Operation:
• Removal of particles and salt crystals, which might potentially damage
the seals
• Lubrication of seal/piston interface
• Cooling of pistons
Seal Wash Operation:
• PERIODIC operation, e.g. 0.5 min every 7 min
• Use 10 % 2- Propanol in water (100 % 2- Propanol for normal phase
applications)
• Position wash solvent bottle above and waste bottle below instrument
• Exchange solvent weekly. Do not recycle seal wash solvent
• Test Peristaltic Pump
Seal Wash Dialog in OpenLab Software (1290 Infinity Pumps only):
• Seal Wash settings are NO method parameters
• Find dialog under CONTROL
• On ERROR – seal wash is switched off
• At Power Off – seal wash is switched off
Seal Wash in 1290 Infinity II Pumps:
• PERIODIC operation for 0.5 min every 7 min when pump is switched on
• Controlled by the seal wash sensor
• Guided out to the waste on the right side

Za odpiranje dokumenta
kliknite tukaj …

Comments are closed.

Politika zasebnosti
Chemass

Naša spletna stran uporablja piškotke za namene zagotavljanja boljše funkcionalnosti in uporabniške izkušnje ter štetja uporabnikov na spletnem mestu. Uporabljamo piškotke Google Analytics, izdane s strani Google inc.. Informacije so shranjene v piškotkih vašega brskalnika in služijo namenu prepoznavanja vaše izbire ter anonimnemu štetju katere Chemassove spletne vsebine so se vam zdele zanimive in ste si jih ogledali.

Uporabo piškotkov lahko prilagodite v nastavitvah spodaj.

Obvezno potrebni piškotki

Priporočamo, da Obvezno potrebne piškotke vedno dovolite, da lahko shranimo vaše nastavitve glede uporabe piškotkov.

Če to možnost onemogočite boste ob vsakem naslednjem obisku Chemassovih strani morali izbiro o piškotkih ponovno nastaviti.

Google Analytics

Spletna stran uporablja Google Analytics za zbiranje anonimnih informacij o številu obiskovalcev in obiskanih straneh.

Če ta piškotek aktivirate nam omogočite učinkovitejše zagotavljanje boljše funkcionalnosti in uporabniške izkušnje na Chemassovi spletni strani.