Calibrating GPC Column – A Guide to Best Practice

Rethinking how and how often we use standards in GPC applications can yield significant improvements to the overall quality of results, including:
Improved reproducibility Improved resolution, leading to
better accuracy Earlier detection of problems Reduced trouble-shooting and system downtime Statistically significant analysis of the system
Introduction Calibration of GPC/SEC column sets using narrow molecular weight standards is a well-established and common technique for the molecular weight distribution of samples. However, analysts sometimes underutilize this technique or do not get the best from it. This paper describes best practice in the calibration of GPC columns, to ensure superior results are achieved in any laboratory. By incorporating the following information into standard operating procedures, every analyst can achieve the many advantages offered when the calibration curve is viewed as an integral component of method development

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