Development of a Method for the Chiral Separation of D/L‑Amphetamine


This Application Note demonstrates method development for the chiral
separation of D/L-amphetamine using an Agilent 1260 Infinity II SFC System. The
Agilent 6495 triple quadrupole MS demonstrates the quantification of these two
chiral enantiomeric forms. The method development process is described, and
the final analytical method was used for the determination of calibration curves
and the limit of quantification using a triple quadrupole MS. The analysis of a
processed authentic whole blood sample is shown.

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