Scaling Small Molecule Purification Methods for HPLC

Reversed-phase liquid chromatography (LC) is often associated with quantitative or analytical-scale analysis, but it is also useful as a preparativescale tool for purification. For any purification project, there are three possible targets: purity, yield, and throughput. These targets are related to one another and are commonly represented as a triangle. Two of these targets can be achieved at the expense of the third. You can clarify your path forward by prioritizing the objectives of your purification project before method development. Za odpiranje dokumenta
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