Reversed-phase liquid chromatography (LC) is often associated with
quantitative or analytical-scale analysis, but it is also useful as a preparativescale tool for purification. For any purification project, there are three
possible targets: purity, yield, and throughput. These targets are related to
one another and are commonly represented as a triangle.
Two of these targets can be achieved at the expense of the third. You can
clarify your path forward by prioritizing the objectives of your purification
project before method development.
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