High-Resolution Sampling 2D-LC with the Agilent 1290 Infinity II 2D-LC Solution

The Agilent 1290 Infinity II 2D-LC solution presents the ability to easily switch
between comprehensive 2D-LC (LCxLC), multiple heart-cutting 2D-LC, or
high-resolution sampling 2D-LC. This Technical Overview shows the benefit of
high-resolution sampling 2D-LC for reliable quantification of substances coeluting
in the first dimension (1D). The quantification of compounds typically contained in
green tea is used as an example. The compounds (+)-catechin and (–)-epicatechin
were separated in the first dimension, and could be quantified based on their
1D peaks using the standard workflow. The compounds (–)-epigallocatechin gallate
and caffeine coelute in the first dimension, but can be separated in the second
dimension. High-resolution sampling 2D-LC ensures that entire 1D peaks can be
transferred to the second dimension (2D) column. Using this technique, a reliable
quantification of the two compounds based on their second-dimension peaks was
possible.

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