Chromatographers working with HPLC and UHPLC are often challenged by problems such as peak tailing, peak broadening, split peaks, carryover, etc. One common cause for those problems that is often overlooked and costs much time in troubleshooting is poor tubing connection. Dead volume or micro-leakage in tubing connections can greatly affect the performance and reproducibility of chromatographic analysis, especially with modern UHPLC and Fast LC columns.
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