Principles And Practical Aspects Of Preparative LC (Primer)

As a synthetic chemist, biologist or engineer, it’s your job to study the impact of chemical compounds. To achieve this goal, you need to stay at the leading edge of your field of research. Consequently, you often find yourself with less time for tasks that are not necessarily your core competences but nevertheless important for your workflows. Isolation and purification of chemical compounds are typical of these tasks. In situations where the compound of interest is not available in pure form, you are challenged to purify it yourself. Possible scenarios include synthesis of the compound in multiple stages, using purification as an interim step, or isolation of the compound from a natural source when synthesis is too complex and tedious. Other scenarios that require you to turn to purification techniques include, for example, when flash chromatography did not yield the desired purity, or when crystallization did not work the way you expected. Isolation of pure compounds was, in fact, the original purpose of liquid chromatography and as such drove the development of separation science during the last century – with close linkages to the discovery of natural sources and new synthetic pathways. The increasing need for high-value compounds deployed as pharmaceuticals, agrochemicals or nutraceuticals, has in turn justified the extra effort required for optimization of purification processes. Today, preparative chromatography is no longer based on guesswork but is founded solidly on a set of well-documented rules to be followed for optimum results. Scouting for appropriate starting conditions, optimizing for speed, yield, and purity are fundamental considerations. The desired sample throughput determines priorities: high yields for a few different samples justify optimization of yield, whereas dealing with large numbers of different samples at the milligram scale demands proper automation. Now, it is all about getting started with preparative liquid chromatography without having to spend time delving deeply into the literature. Although a primer will never replace textbooks on preparative liquid chromatography to gain a full understanding of the theoretical background, this publication nevertheless bridges the gap between textbook literature and a typical system’s user documentation that provides specific guidance on how to achieve optimum results. Za odpiranje dokumenta
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