The transfer of conventional LC methods from legacy equipment to new instrumentation is an important topic in all laboratories. This Application Note shows the transfer of a conventional LC method for the analysis of antihistaminic drugs from an Agilent 1100 Series Quaternary LC to an Agilent 1260 Infi nity II LC, and proves that equivalent results in terms of retention times and resolution are obtained. Also, the conventional LC method is transferred to UHPLC conditions optimized for resolution as well as for speed using the 1260 Infi nity II LC. This offers the possibility of increasing peak resolution, while enabling time and solvent savings.
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