Quantification of Testosterone in Serum by Liquid Chromatography‑Tandem MS

An ultra-sensitive quantitative analytical method is required for measuring
the concentration of total and free testosterone found in men, and particularly
the low levels found in women and children. Therefore, an analytical method
was developed on an Agilent 6495 Ion Funnel Mass Spectrometer to quantify
and characterize testosterone in serum, and to ascertain the most appropriate
analytical method for laboratory use.

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