Simplified DV200 Evaluation with the Agilent 2100 Bioanalyzer System

Quality assessment of RNA samples is crucial for the success of library preparation for
RNA sequencing workflows. The Agilent 2100 Bioanalyzer system enables the highly
reproducible evaluation of RNA sample quantity and integrity, with the Total RNA Nano
and Pico assays. In general, the RNA integrity number (RIN) is a suitable parameter to
assess sample integrity for RNA sequencing. The establishment of RNA sequencing
approaches for samples originating from formalin-fixed paraffin-embedded (FFPE) tissue
created the need for further differentiation of degraded RNA samples by fragment size
distribution. The DV200 qualitymetric represents the percentage of RNA fragments above
200 nucleotides and shows a high correlation to the precapture library yield of FFPE
samples.
This Technical Overview describes the simplified evaluation of DV200 using the
DV200 RNA Nano and DV200 RNA Pico assays with the Agilent 2100 Bioanalyzer
system.
Primary modifications of the DV200 RNA Nano and Pico assays are:
• Predefined DV200 region
• DV200 presented in region table by default
• Result flagging according to the DV200 range
• DV200 range displayed in chip summary

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