Stem Cell Research (zbirka aplikacij)

MONITOR CELL FATE TRANSITIONS
Improve Differentiation and Reprogramming Outcomes
Cellular age and origin, in addition to donor variability, protocol differences, growth rates and media choices all
contribute to inconsistent reprogramming and/or differentiation efficiencies. Metabolic energy utilization, characterized
before and after cell fate changes occur, identifies the metabolic phenotype and enables researchers to predict and
confirm cell function, revealing actionable reprogramming and differentiation potential.
Adult Fibroblast Cells Stem Cells Hematopoietic Cells Adipocytes
Hepatocytes
Cardiomyocytes
Neural Cells
Differentiation
(iPS/ES Cells)
Reprogramming
(iPS Cells)
Cellular metabolic phenotyping measures
the cell’s energy requirement and pathway
preference for readying the transition between
undifferentiated and differentiated states.
Metabolic switching occurs rapidly as cells
transition from quiescent to pluripotent and/or
from pluripotent to differentiated.
Seahorse XF Technology:
• Live cell
• Real-time
• Label-free
• Dynamic injection ports
• Measures oxygen consumption and
glycolytic rates simultaneously

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