Agilent Glycan Peak Assignment Tool (GPAT)

GPAT is a chromatographic peak assignment tool for N-glycan separations performed with the Agilent AdvanceBio Amide HILIC column in the 2.1 x 150 mm dimension. The tool predicts HILIC retention times for common N-glycans by calibrating liquid chromatography (LC) systems with a commercial glucose ladder to establish a relationship between glucose unit (GU) values and retention time (RT), as described in the literature. [1] GPAT then uses the GU-RT relationship to convert glycan GU values from an internal library into predicted retention times for the LC system. Za odpiranje dokumenta
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