Assessing Integrity of Insect RNA

Assessing total RNA integrity is important for the success of downstream RNA
applications. The Agilent 2100 Bioanalyzer system with the RNA Integrity Number
(RIN) provides a quantitative measure of RNA degradation. Although RINs may not
be ascertained for RNA from all organisms, namely those with unusual or complex
RNA profiles, this Application Note demonstrates the applicability of total RNA
quality assessment of different insect species with the 2100 Bioanalyzer system
and its relevance to downstream quantitative RT-PCR experiments

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